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KMID : 0360319950270020209
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Journal of Korean Cancer Research Association
1995 Volume.27 No. 2 p.209 ~ p.222
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Metastatic Potential of Human Colon Cancer Cell Lines, LoVo and SW480
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설지영
송인상/장일성/배진선/윤완희
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Abstract
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Metastatic spread of malignancy is the main cause of the treatment failure and subsequent death in cancer patients. Recent findings indicate that tumors consist of cell populations with various degrees of metastatic potential, and that selective
growth
of subpopulations with higher metastatic potentials results in metastasis. The purpose of this study was to examine the metastatic potential of human colon cancer cell lines, LoVo and SW480 and to identify some cellular and biochemical
determinants
of
metastasis using various properties which reflect events in metastatic process. The binding activity to ELAM-1, cell-cell aggregation rate, and total MMP activity mere significantly higher in LoVo cells relative to SW480 cells. To define the
effects of
these cellular and biochemical properties on the process of invasion and metastasis of colon cancer cells, in vitro invasion and in vivo metastasis assay were performed. LoVo cells had an enhanced ability to penetrate a reconstituted basement
membrane
to a significantly greater degree than SW480 cells. The capacity to produce tumor nodules in the liver of nude mice subsequent to intrasplenic injection of cells was greater in LoVo cells than in SW480 cells. Also increased nm23-H1 protein
expression
was observed in SW480 cells in which no gross liver metastases were found. The results of this study suggest that LoVo cells have higher liver metastatic potential than SW480 cells and among various biochemical properties ELAM-1 binding and total
MMP
activity are significantly implicated in invasion and metastasis of colon cancer cells. And homotypic cell adhesion and downregulated nm23 gene product may be important in the metastatic cascade, but their roles should be further elucidated.
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KEYWORD
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